Mode of action
Genes (DNA) are transcribed to mRNA in the nucleus. This single-stranded mRNA is transported to the cytoplasm where it is translated by ribosomes into proteins.
Short complementary DNA-oligonucleotides (asODN) can bind specifically to a targeted mRNA leading to their functional inactivation and enzymatical destruction.
Antisense-oligonucleotides (asODN) are not produced by the cell itself and have to be delivered externally.
Inside cells asODN have a half live that is defined by many factors including its chemical modifications.
Due to its tunable turn-over and applied doses, antisense action can be controlled.